CST, 3794S, SKAR alpha/beta Antibody

Polyclonal Antibody for studying SKAR. Validated for Western Blotting. Highly specific and rigorously validated in-house, SKAR alpha/beta Antibody (CST #3794) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SKAR alpha/beta Antibody detects endogenous levels of total SKAR protein. This antibody recognizes both the α and the β isoforms. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino terminal residues of human SKAR. Antibodies are purified using protein A and peptide affinity chromatography. Background p70 S6 kinase is a mitogen activated Ser/Thr protein kinase downstream of phosphoinositide-3 kinase (PI3K) and the target of rapamycin, FRAP/mTOR. p70 S6 kinase is required for cell growth and cell cycle progression (1,2). SKAR is a recently discovered substrate of S6K1. SKAR exists in two isoforms, α and β, the latter having a 29 amino acid truncation. Phosphorylation of SKAR is mitogen-induced and sensitive to rapamycin. Reduction in SKAR protein levels results in decreased cell size, further implicating SKAR in cell growth control (3). Alternate Names 46 kDa DNA polymerase delta interaction protein; DNA polymerase delta interacting protein 3; KIAA1649; p46; PDIP3; PDIP46; POLDIP3; polymerase (DNA-directed), delta interacting protein 3; polymerase (DNA) delta interacting protein 3; polymerase delta interacting protein 46; Polymerase delta-interacting protein 3; RNA-binding protein P46; S6K1 Aly/REF-like target; SKAR Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 46, 43 SOURCE: Rabbit