CST, 3795S, Frizzled5 Antibody

Polyclonal Antibody for studying FZD5. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Frizzled5 Antibody (CST #3795) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Frizzled5 Antibody detects transfected levels of total frizzled5 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with synthetic peptide corresponding to the amino acid sequence surrounding Ala550 of human frizzled5. Antibodies are purified by protein A and peptide affinity chromatography. Background Frizzled (Fzd) belongs to the seven transmembrane-spanning G-protein-coupled receptor (GPCR) superfamily (1). Fzds have a large extracellular N-terminal region containing a cysteine-rich domain (CRD), which is involved in binding to Wnt proteins (1,2). The intracellular C-terminus binds to the PDZ domain of Dvl proteins, a major signaling component downstream of Fzd (3). Wnt proteins bind to Fzd and the co-receptors LRP5 or LPR6, and activate Wnt/β-catenin pathway through inhibiting phosphorylation of β-catenin by GSK3-β (4,5). In addition to this canonical Wnt/β-catenin pathway, some Wnt proteins can also activate the Fzd/Ca pathway and Fzd/PCP (planar cell polarity) pathway (6,7). The mammalian Fzd subfamily has 10 members (Fzd1 to Fzd10) and they may mediate signaling through different pathways (8). Some Fzds can also bind to other secreted proteins, like Norrin and R-Spondin (9-11). Alternate Names C2orf31; DKFZp434E2135; frizzled 5, seven transmembrane spanning receptor; frizzled class receptor 5; frizzled family receptor 5; frizzled homolog 5 (Drosophila); Frizzled-5; Fz-5; FZD5; FzE5; hFz5; MGC129692; seven-transmembrane receptor frizzled-5; Wnt receptor Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 50-65 SOURCE: Rabbit