CST, 38013S, Phospho-Fgr (Tyr412) (E3R7L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FGR (Tyr412) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Fgr (Tyr412) (E3R7L) Rabbit Monoclonal Antibody (CST #38013) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Fgr (Tyr412) (E3R7L) Rabbit Monoclonal Antibody recognizes endogenous levels of Fgr protein only when phosphorylated at Tyr412. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr412 of human Fgr protein. Background Fgr (c-Fgr kinase) is a member of the Src tyrosine kinase family. It has a membrane-associated amino-terminal domain that is highly divergent from other family members, internal conserved SH2 and SH3 domains, and a highly conserved carboxy-terminal tyrosine kinase catalytic domain (1,2). Tyrosine 412 is located in the activation loop; autophosphorylation of this residue is critical for the activation of Fgr tyrosine kinase activity, and is counter-regulated by Src-mediated phosphorylation at Tyr523 (3). c-Fgr is predominantly expressed in cells of hematopoietic origin, including differentiated myeloid cells, NK, and B cells (4,5). Fgr plays an important role in the signaling cascade from membrane receptors lacking intrinsic tyrosine kinase activity, such as Bcr, FcR, and the integrin family of receptors (6). It was demonstrated that Fgr functions as a selective inhibitor of beta 2 integrin-mediated signaling and Syk kinase function in monocytes (6). Alternate Names c-fgr; c-fgr protooncogene; c-src-2 proto-oncogene; c-src2; feline Gardner-Rasheed sarcoma viral oncogene homolog; FGR; FGR proto-oncogene, Src family tyrosine kinase; FLJ43153; Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog; MGC75096; p55-c-fgr protein; p55-Fgr; p55c-fgr; p58-Fgr; p58c-fgr; Proto-oncogene c-Fgr; proto-oncogene tyrosine-protein kinase FGR; SRC2; Tyrosine-protein kinase Fgr; v-fgr feline Gardner-Rasheed sarcoma viral oncogene homolog Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 56 Source/Isotype: Rabbit IgG