CST, 3803S, PPIG Antibody

Polyclonal Antibody for studying PPIG. Validated for Western Blotting. Highly specific and rigorously validated in-house, PPIG Antibody (CST #3803) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PPIG Antibody detects endogenous levels of total PPIG protein. This antibody also recognizes a unidentified protein at 49 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu391 of human PPIG. Antibodies are purified by peptide affinity chromatography. Background PPIG belongs to a highly conserved class of cyclophilins that function as peptidyl-prolyl-isomerases (PPIases) to catalyze the conversion of cis-proline to trans-proline in a polypeptide chain (1-4). PPIG contains an amino-terminal cyclophilin domain followed by Nopp140 repeats that are involved in its function as a nuclear chaperone (5). The carboxy-terminal of PPIG contains a SR (arginine-serine dipeptide repeat) domain (3,4) that is involved in pre-mRNA splicing and processing (6). PPIG interacts with the carboxy-terminal domain of RNA polymerase II as well as several other SR family splicing factors. These interactions lead to changes in localization and conformation and suggest a regulatory role in transcription and pre-mRNA splicing in the elongating RNA polymerase complex (7,8). PPIG is found in the nuclear matrix and nuclear speckles and is involved in the regulation of gene expression. PPIG shows a predominantly diffuse cytoplasmic distribution at the onset of mitosis, and in late telophase the isomerase is recruited to the newly formed nuclei (9). Alternate Names CARS-cyclophilin; CARS-Cyp; CASP10; Clk-associating RS-cyclophilin; Cyclophilin G; CYP; MGC133241; Peptidyl-prolyl cis-trans isomerase G; Peptidyl-prolyl isomerase G; peptidyl-prolyl isomerase G (cyclophilin G); peptidylprolyl isomerase G; peptidylprolyl isomerase G (cyclophilin G); PPIase G; PPIG; Rotamase G; SCAF10; SR-cyclophilin; SR-cyp; SR-related CTD-associated factor 10; SRcyp Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 110 SOURCE: Rabbit