CST, 3806S, Phospho-Rictor (Thr1135) (D30A3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Rictor (Thr1135) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Rictor (Thr1135) (D30A3) Rabbit Monoclonal Antibody (CST #3806) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Rictor (Thr1135) (D30A3) Rabbit Monoclonal Antibody detects endogenous levels of rictor protein only when phosphorylated at Thr1135. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Thr1135 of human Rictor protein. Background Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4). Phosphorylation of Thr1135 on rictor was identified at Cell Signaling Technology (CST) using PhosphoScan , CST's LC-MS/MS platform for phosphorylation site discovery (5). Additional research indicates that rictor is phosphorylated at Thr1135 by p70 S6K, which negatively regulates mTORC2 protein complex as part of a negative feedback mechanism controlling Akt activity (6-8). Alternate Names AVO3; AVO3 homolog; DKFZp686B11164; hAVO3; KIAA1999; mAVO3; MGC39830; PIA; pianissimo; Rapamycin-insensitive companion of mTOR; RICTOR; RICTR; RPTOR independent companion of MTOR complex 2; RPTOR independent companion of MTOR, complex 2; TORC2-specific protein AVO3 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG