CST, 3810S, Enolase-1 Antibody

Polyclonal Antibody for studying Enolase1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Enolase-1 Antibody (CST #3810) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Enolase-1 Antibody detects endogenous levels of total enolase-1 protein and does not corss-react with enolase-2. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Background Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (α-enolase), enolase-2 (γ-enolase) and enolase-3 (β-enolase) that can form both homo- and heterodimers. Expression of the enolase isoforms differs in a tissue specific manner (1). Enolase-1 plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion (2,3). Abnormal expression of enolase-1 is associated with tumor progression in some cases of breast and lung cancer (4-7). Alternatively, an enolase-1 splice variant (MBP-1) binds the c-myc promoter p2 and may function as a tumor suppressor. For this reason enolase-1 is considered as a potential therapeutic target in the treatment of some forms of cancer (8). Alternate Names 2-phospho-D-glycerate hydro-lyase; alpha enolase like 1; Alpha-enolase; C-myc promoter-binding protein; c-myc promoter-binding protein-1; ENO1; ENO1L1; ENOA; enolase; Enolase 1; enolase 1, (alpha); enolase-alpha; epididymis secretory protein Li 17; HEL-S-17; MBP-1; MBPB1; MPB-1; MPB1; MYC promoter-binding protein 1; NNE; Non-neural enolase; Phosphopyruvate hydratase; Plasminogen-binding protein; PPH; tau-crystallin Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 47 SOURCE: Rabbit