CST, 3811S, PI3 Kinase Class III Antibody

Polyclonal Antibody for studying PIK3C3. Validated for Western Blotting. Highly specific and rigorously validated in-house, PI3 Kinase Class III Antibody (CST #3811) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PI3 Kinase Class III Antibody detects endogenous levels of total PI3K class III protein. This antibody may cross-react with an unidentified protein at ~55 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human PI3K class III. Antibodies are purified peptide affinity chromatography. Background Three distinct types of phosphoinositide 3-kinases (PI3K) have been characterized. Unlike other PI3Ks, PI3K class III catalyzes the phosphorylation of phosphatidylinositol at the D3 position, producing phosphatidylinositol-3-phosphate (PIP3) (1). PI3K class III is the mammalian homolog of Vps34, first identified in yeast. PI3K class III interacts with the regular subunit p150, the mammalian homolog of Vps15, which regulates cellular membrane association through myristoylation (2,3). PIP3 recruits several proteins with FYVE or PX domains to membranes regulating vesicular transport and protein sorting (4). Moreover, PI3K class III has been shown to regulate autophagy, trimeric G-protein signaling, and the mTOR nutrient-sensing pathway (5). Alternate Names hVps34; MGC61518; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; phosphoinositide-3-kinase, class 3; PI3-kinase type 3; PI3K type 3; PIK3C3; PK3C3; PtdIns-3-kinase type 3; VPS34 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100 SOURCE: Rabbit