CST, 3812S, LSP1 Antibody

Polyclonal Antibody for studying Lsp1. Validated for Western Blotting. Highly specific and rigorously validated in-house, LSP1 Antibody (CST #3812) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LSP1 Antibody detects endogenous levels of LSP1 protein independent of phosphorylation. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 37-48 of human LSP1. Antibodies are purified by protein A and peptide affinity chromatography. Background LSP1 is a 52 kDa leukocyte-specific protein expressed in B cells, T cells, macrophages and neutrophils (1). The protein binds F-actin through its C-terminal actin-binding repeat and regulates cytoskeletal-related cellular movement such as polarization, chemotaxis and locomotion (2-4). LSP1 is a phospho-protein and has been identified as a major substrate of MAP kinase and PKC in lymphocytes and neutrophils (5-7). The phosphorylation is possibly related to its cytoskeletal regulatory function. Alternate Names 47 kDa actin binding protein; 47 kDa actin-binding protein; 52 kDa phosphoprotein; F-actin binding and cytoskeleton associated protein; leufactin (leukocyte F-actin binding protein); leukocyte-specific protein 1; LSP1; lymphocyte specific protein 1; Lymphocyte-specific antigen WP34; Lymphocyte-specific protein 1; lymphocyte-specific protein pp52; pp52; WP34 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 52 SOURCE: Rabbit