CST, 3813S, Plexin A1 Antibody

Polyclonal Antibody for studying PLXNA1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Plexin A1 Antibody (CST #3813) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Plexin A1 Antibody detects endogenous levels of total Plexin A1 protein. Species Reactivity: Human, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Gln1875 of human Plexin A1. Antibodies are purified by peptide affinity chromatography. Background Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single membrane-spanning signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3). Alternate Names DKFZp761P19121; NOV; NOVP; plexin 1; plexin A1; Plexin-A1; PLXA1; PLXN1; PLXNA1; Semaphorin receptor NOV Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 211 SOURCE: Rabbit