CST, 38142SF, BST2 (E4N3W) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying BST2. Validated for WB,IHC,IHC,F. Highly specific and rigorously validated in-house, BST2 (E4N3W) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #38142) is ready to ship. Product Usage Information This product is the carrier free version of product #95940. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 95940 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity BST2 (E4N3W) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total BST2 protein. This clone shows a preference for the BST2 dimer by western blot. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human BST2 recombinant protein. Background BST2 (CD317, Tetherin, HM1.24) is a type II transmembrane glycoprotein functioning as a major mediator of the innate immune defense against the dissemination of enveloped viruses by tethering virion on the cell surface (1). BST2 has an N-terminal cytoplasmic tail for endocytosis and cytoskeletal signaling, a transmembrane domain, an extracellular domain containing putative disulfide bonds and coiled-coil region for homodimer formation, and a C-terminal GPI domain for membrane anchoring (2,3). Both the transmembrane domain and the GPI domain can insert either into the cell membrane or the viral envelope membrane and hold them together to prevent viral release. Some viruses encode proteins, such as HIV-1 and Vpu, respectively, to act as antagonists to counteract BST2 (2,3). BST2 is overexpressed in gastrointestinal cancers, breast cancer, lung cancer, and multiple myeloma (4-7). BST2 monoclonal antibody targeting myeloma or lung cancer cells induces cellular cytotoxicity and cell death (ADCC, antibody-dependent cell-mediated cytotoxicity). Thus, BST2 serves as a potential target for tumor immunotherapy. Alternate Names Bone marrow stromal antigen 2; bone marrow stromal cell antigen 2; BST-2; BST2; CD317; HM1.24 antigen; NPC-A-7; Tetherin Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28-40, 50-70 Source/Isotype: Rabbit IgG