CST, 3816T, Plexin A4 (C5D1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Plexin A4. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Plexin A4 (C5D1) Rabbit Monoclonal Antibody (CST #3816) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Plexin A4 (C5D1) Rabbit Monoclonal Antibody detects endogenous levels of total Plexin A4 protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a GST-fusion protein corresponding to residues from the human sequence of Plexin A4. Background Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single membrane-spanning signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3). Both Plexin A3 and A4 mediate the responses to class 3 Semaphorins in sensory and sympathetic neurons. In particular, Plexin A4 is responsible for signaling of Sema3A via neuropilin-1, while Plexin A3 is responsible for signaling of Sema3F via neuropilin-2 (4). Alternate Names DKFZp434G0625; DKFZp566O0546; epididymis secretory sperm binding protein; FAYV2820; FLJ35026; FLJ38287; KIAA1550; PLEXA4; plexin A4; Plexin-A4; PLXA4; PLXNA4; PLXNA4A; PLXNA4B; PRO34003 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 212 Source/Isotype: Rabbit IgG