CST, 38186SF, TMPRSS4 (E5F4W) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying TMPRSS4. Validated for Western Blotting. Highly specific and rigorously validated in-house, TMPRSS4 (E5F4W) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #38186) is ready to ship. Product Usage Information This product is the carrier free version of product #84382. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 84382 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity TMPRSS4 (E5F4W) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total TMPRSS4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TMPRSS4 protein. Background Transmembrane protease serine 4 (TMPRSS4) is a type-II transmembrane glycoprotein that functions as a serine protease. The extracellular region of TMPRSS4 harbors a serine protease catalytic domain and a scavenger receptor domain, which is implicated in lipid binding (1,2). TMPRSS4 activity has been implicated in viral infections through its ability to regulate the proteolytic processing of the influenza virus hemagglutinin protein and SARS-CoV-2 spike protein (3,4). TMPRSS4 is also implicated in cancer cell metastasis through the cleavage of urokinase-type plasminogen activator and activation of intracellular signaling cascades that promote cell migration (5,6). These findings, combined with the observation that TMPRSS4 is overexpressed in multiple types of human solid tumors, have prompted investigation into therapeutically targeting TMPRSS4 protease activity (6-9). Alternate Names CAPH2; Channel-activating protease 2; Membrane-type serine protease 2; MT-SP2; TMPRSS3; TMPRSS4; TMPS4; Transmembrane protease serine 4; transmembrane protease, serine 4; transmembrane serine protease 3; transmembrane serine protease 4; type II membrane serine protease Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 62, 55 Source/Isotype: Rabbit IgG