CST, 38199T, FANCM (E5Y9H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FANCM. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, FANCM (E5Y9H) Rabbit Monoclonal Antibody (CST #38199) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FANCM (E5Y9H) Rabbit Monoclonal Antibody recognizes endogenous levels of total FANCM protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human FANCM protein. Background Fanconi anemia (FA) is an autosomal recessive genetic disorder resulting in symptoms that include chromosomal breakage, bone marrow failure, hypersensitivity to DNA cross-linking agents (such as mitomycin C), and a predisposition to cancer (1). FANCB is an X-linked member of the FA nuclear complex (FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCM). In response to DNA damage, the FA nuclear complex induces mono-ubiquitination of FANCD2 and FANCI (2). FANCJ/BRIP1, FANCD1/BRCA2, and FANCN/PALB2 are then recruited to sites of DNA damage along with other DNA repair proteins. FA signaling is important in maintenance of chromosome stability and control of mitosis (3). Studies of FANCB knockout embryonic stem cells suggest a role for FANCB in the formation of Rad51 and FANCD2 foci at chromosomal sites of DNA damage (4). Alternate Names ATP-dependent RNA helicase FANCM; FA complementation group M; FAAP250; FANCM; Fanconi anemia complementation group M; Fanconi anemia group M protein; Fanconi anemia-associated polypeptide of 250 kDa; Fanconi anemia, complementation group M; KIAA1596; MGC176453; POF15; Protein FACM; Protein Hef ortholog; SPGF28 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 230 Source/Isotype: Rabbit IgG