CST, 38216SF, P2Y12 (E9J1J) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying P2Y12 mouse. Validated for Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, P2Y12 (E9J1J) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #38216) is ready to ship. Product Usage Information This product is the carrier free version of product #69766. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 69766 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity P2Y12 (E9J1J) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total P2Y12 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro173 of mouse P2Y12 protein. Background Purinergic signaling involves cell signaling pathways that are activated by nucleosides and nucleotides via specific purinergic receptors (1). Purinergic receptors include two large families, P1 receptors that are activated by nucleosides and P2 receptors that are activated by nucleotides. P2 receptors are further divided into ionotropic P2X receptors and metabotropic P2Y receptors. P2Y purinoceptor 12 (P2Y12) is a member of the P2Y receptor family (2). As G protein-coupled receptors, P2Y12 are activated by ADP and ATP to drive downstream second messenger pathways. P2Y12 is required for normal platelet aggregation and blood coagulation (3). In addition to their role in thrombosis, P2Y12 receptors drive purinergic signaling in the central nervous system (CNS). P2Y12 receptors are enriched in microglia and regulate nucleotide-dependent microglia chemotaxis and morphology (4). Furthermore, P2Y12 receptors regulate specialized microglia/neuron junctions that provide microglia-dependent protection of neuronal functions in response to brain injury and disease (5). Alternate Names 2900079b22rik; 4921504d23rik; p2ry12; P2Y purinoceptor 12; P2Y1; p2y12; purinergic receptor p2y, g-protein coupled 12; purinergic receptor p2y12 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG