CST, 3822S, MST4 Antibody

Polyclonal Antibody for studying MST4. Validated for Western Blotting. Highly specific and rigorously validated in-house, MST4 Antibody (CST #3822) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MST4 Antibody recognizes endogenous levels of total MST4 protein independent of phosphorylation. This antibody does not cross-react with MST1-3 proteins. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of human MST4 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Mammalian sterile-20-like (MST) kinases are upstream regulators of mitogen-activated protein kinase (MAPK) signaling pathways that regulate multiple biological processes, including apoptosis, morphogenesis, cell migration, and cytoskeletal rearrangements (1). This group of serine/threonine kinases includes a pair of closely related proteins (MST1, MST2) that are functionally distinct from the more distantly related MST3 and MST4 kinases. All four MST kinases share a conserved amino-terminal kinase domain and carboxy-terminal regulatory and interaction domains (1-3). At least three of these kinases (MST1-3) promote apoptosis and are activated by caspase cleavage followed by nuclear translocation of the active kinase. MST1/2 kinases play a key role in the Hippo signaling pathway, an evolutionarily conserved program that controls organ size by regulating cell proliferation, apoptosis, and stem cell self renewal (4). Mammalian Sterile 20-like kinase 4 (MST4, STK26, MASK) is a Golgi-localized kinase that is cleaved by caspase-3 . While its potential role in apoptosis is unclear, research studies indicate that MST4 is involved in MAPK and EGF pathway signaling (5,6). MST4 and the serine/threonine kinase YSK1 (STK25) localize to the Golgi apparatus following association with the Golgi scaffold protein GM130. Binding to GM130 activates MST4 through autophosphorylation at Thr178 (7). Alternate Names Mammalian STE20-like protein kinase 4; mammalian sterile 20-like 4; MASK; MST-4; Mst3 and SOK1-related kinase; MST4; serine/threonine kinase 26; serine/threonine protein kinase 26; serine/threonine protein kinase MASK; serine/threonine protein kinase MST4; Serine/threonine-protein kinase 26; Serine/threonine-protein kinase MASK; Serine/threonine-protein kinase MST4; STE20-like kinase 4; STE20-like kinase MST4; STK26 Specification REACTIVITY: H M R Mk B SENSITIVITY: Endogenous MW (kDa): 52 SOURCE: Rabbit