CST, 38276S, BLVRA (F7Z7R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BVR. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, BLVRA (F7Z7R) Rabbit Monoclonal Antibody (CST #38276) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity BLVRA (F7Z7R) Rabbit Monoclonal Antibody recognizes endogenous levels of total BLVRA protein. This antibody detects a 120 kDa band of unknown origin in some cell lines. Non-specific staining was observed in human skeletal muscle by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly91 of human BLVRA protein. Background Biliverdin reductase A (BLVRA or BVRA) is responsible for the reduction of biliverdin, a product of heme oxygenase, to produce bilirubin, the end product of the heme catabolic pathway and major physiological antioxidant (1). In addition to its canonical role as an antioxidant, bilirubin acts as a powerful immunosuppressant and has hormonal activity that links it to a number of signaling pathways (reviewed in 2-4). Altered levels of bilirubin and BVRA have been reported in many pathological conditions, including cancer, cardiovascular, metabolic, and neurodegenerative diseases. Deletion of BLVRA leads to an increase in oxidative stress and autophagy impairment (5,6). BVRA has been shown to be a substrate of the insulin receptor and also phosphorylates insulin receptor substrate 1 (IRS-1) to regulate the insulin signaling pathway (7). Reduced BVRA expression is associated with changes in insulin signaling during obesity (8). BVRA phosphorylation of glycogen synthase kinase-3β (GSK-3β) inhibits its activity and phosphorylation of peroxisome proliferator-activated receptor α (PPARα), which protects from hepatic steatosis (9). Impairment of BVRA has been associated with Alzheimer's disease (10-12). Levels of BVRA are frequently elevated in cancer, where it regulates several key mitogenic pathways (reviewed in 13). Alternate Names BIEA; Biliverdin reductase A; Biliverdin-IX alpha-reductase; BLVR; BLVRA; BVR; BVR A; BVRA; testis tissue sperm-binding protein Li 61n Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 38 Source/Isotype: Rabbit IgG