CST, 3831S, Phospho-PLD1 (Thr147) Antibody

Polyclonal Antibody for studying PLD1 (Thr147) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-PLD1 (Thr147) Antibody (CST #3831) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-PLD1 (Thr147) Antibody detects endogenous levels of PLD1 only when phosphorylated at threonine 147. This antibody does not cross-react with phosphorylated PLD2. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr147 of human PLD1. Antibodies are purified by protein A and peptide affinity chromatography. Background Phosphatidylcholine-specific phospholipase D (PLD) hydrolyzes phosphatidylcholine (PC) to produce choline and phosphatidic acid (PA). PA is the precursor of the second messenger, diacylglycerol (DAG). Two isoforms of PLD (PLD1 and PLD2) have been identified so far. Both are regulated by protein kinases, small GTPases and Ca2 (1). PLD1 is phosphorylated at Ser2, Ser561, and Thr147 by PKC (2,3). Phosphorylation at Thr147 and Ser561 regulates PLD1 activity (3). Alternate Names Choline phosphatase 1; CVDD; hPLD1; Phosphatidylcholine-hydrolyzing phospholipase D1; Phospholipase D1; phospholipase D1, phophatidylcholine-specific; phospholipase D1, phosphatidylcholine-specific; PLD 1; PLD1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 120 SOURCE: Rabbit