CST, 38355SF, p27 Kip1 (D37H1) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying p27 Kip1. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, p27 Kip1 (D37H1) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #38355) is ready to ship. Product Usage Information This product is the carrier free version of product #3688. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 3688 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity p27 Kip1 (D37H1) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total p27 Kip1 protein. This antibody may cross-react with p57 Kip2. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p27 Kip1. Background p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4). Alternate Names CDKN1B; CDKN4; CDN1B; cyclin dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; KIP1; MEN1B; MEN4; p27Kip1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 27 Source/Isotype: Rabbit IgG