CST, 38410S, SNF2H (D4W6N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SNF2H. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SNF2H (D4W6N) Rabbit Monoclonal Antibody (CST #38410) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SNF2H (D4W6N) Rabbit Monoclonal Antibody recognizes endogenous levels of total SNF2H protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala65 of human SNF2H protein. Background Sucrose nonfermenting 2 homolog (SNF2H, SMARCA5) is one of two orthologs of the ISWI (imitation switch) ATPases encoded by the mammalian genome (1). SNF2H is part of the SNF2 family of chromatin remodeling factors that use ATP hydrolysis to catalyze biochemical reactions in several mammalian chromatin-remodeling complexes, including ACF1, RSF1, CHRAC, NoRC, WSTF, and WCRF180 (2). Research studies show that SNF2H is crucial for chromatin organization, DNA damage response, and differentiation (1-7). The SNF2H helicase facilitates DNA damage repair by actively moving nucleosomes for DNA damage response (DDR) proteins to effectively associate with damaged regions (3). Additional studies show that repair of double stranded breaks (DSBs) significantly decreases in the absence of SNF2H (3), and these cells become highly sensitive to DNA damage caused by x-rays and chemical treatments inducing DSBs (4,5). Alternate Names hISWI; hSNF2H; ISWI; SMARCA5; SMCA5; SNF2H; Sucrose nonfermenting protein 2 homolog; sucrose nonfermenting-like 5; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; WCRF135 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 125 Source/Isotype: Rabbit IgG