CST, 3842S, LIMK1 Antibody

Polyclonal Antibody for studying LIMK1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, LIMK1 Antibody (CST #3842) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity LIMK1 Antibody detects endogenous levels of total LIMK1 protein independent of phosphorylation. This antibody does not cross-react with LIMK2. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human LIMK1. Antibodies are purified by protein A and peptide affinity chromatography. Background LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7). Alternate Names LIM domain kinase 1; LIM motif-containing protein kinase; LIMK; LIMK-1; LIMK1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70 SOURCE: Rabbit