CST, 3845S, LIMK2 (8C11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LIMK2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, LIMK2 (8C11) Rabbit Monoclonal Antibody (CST #3845) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LIMK2 (8C11) Rabbit Monoclonal Antibody detects endogenous levels of total LIMK2 protein. This antibody does not cross-react with LIMK1. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human LIMK2. Background LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7). Alternate Names LIM domain kinase 2; LIMK-2; LIMK2 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG