CST, 3847S, E2-25K/Hip2 Antibody

Polyclonal Antibody for studying HIP2. Validated for Western Blotting. Highly specific and rigorously validated in-house, E2-25K/Hip2 Antibody (CST #3847) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity E2-25K/Hip2 Antibody detects endogenous levels of total E2-25K/Hip2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg11 of human E2-25K/Hip2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1). E2-25K (Hip2) is a member of the E2 protein family that catalyzes multiubiquitin chain synthesis via Lys48 of ubiquitin (2). E2-25K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-β, huntingtin, and MHC-heavy chain proteins (3-5). Lys14 of E2-25K can be modified by SUMOylation, with this modification resulting in inhibited E2 activity (6). Alternate Names DKFZp564C1216; DKFZp686J24237; E2 ubiquitin-conjugating enzyme K; E2-25K; E2(25K); HIP-2; HIP2; huntingtin interacting protein 2; Huntingtin-interacting protein 2; HYPG; LIG; UBC1; UBE2K; Ubiquitin carrier protein; ubiquitin conjugating enzyme E2 K; ubiquitin conjugating enzyme E2K; Ubiquitin-conjugating enzyme E2 K; Ubiquitin-conjugating enzyme E2-25 kDa; Ubiquitin-conjugating enzyme E2-25K; Ubiquitin-conjugating enzyme E2(25K); ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); Ubiquitin-protein ligase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 25 SOURCE: Rabbit