CST, 3848S, UBE2L3 Antibody

Polyclonal Antibody for studying UBE2L3. Validated for Western Blotting. Highly specific and rigorously validated in-house, UBE2L3 Antibody (CST #3848) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity UBE2L3 Antibody detects endogenous levels of total UBE2L3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys145 of human UBE2L3 protein. Antibodies are purified by peptide affinity chromatography. Background Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1). UBCH7 (also called UBE2L3) is an 17 kDa ubiquitin-conjugating enzyme E2 (3). It can interact with different E3 ligase including E6-AP, SRC-1, c-Cbl, parkin and mediates regulation of a wide variety signaling pathways and is associated with diseases such as Parkinson's disease (4-7). Alternate Names E2 ubiquitin-conjugating enzyme L3; E2-F1; L-UBC; UB2L3; UBCE7; UBCH7; UbcM4; UBE2L3; Ubiquitin carrier protein L3; ubiquitin conjugating enzyme E2 L3; ubiquitin conjugating enzyme E2L 3; Ubiquitin-conjugating enzyme E2 L3; Ubiquitin-conjugating enzyme E2-F1; ubiquitin-conjugating enzyme E2L 3; ubiquitin-conjugating enzyme UBCH7; Ubiquitin-protein ligase L3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 17 SOURCE: Rabbit