CST, 38515S, CD96 (F2I1M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD96. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CD96 (F2I1M) Rabbit Monoclonal Antibody (CST #38515) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CD96 (F2I1M) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD96 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracullular domain of human CD96 protein. Background Cluster of Differentiation 96 (CD96), also known as T cell-activated increased late expression protein (TACTILE), is a heavily glycosylated type I transmembrane protein belonging to the immunoglobulin superfamily (1-3). CD96 is expressed on peripheral T cells and natural killer (NK) cells, and is strongly upregulated on these cell types after activation (1,4). It is also expressed on transformed T cells, and at very low levels on activated B cells (1,5,6). CD96 interacts with the receptor ligand PVR (CD155), and is involved in adhesion of activated T and NK cells to target cells during the late phase of the immune response (2,4). CD96 competes with DNAM-1 (CD226) for binding to PVR (CD155) in order to negatively regulate T and NK cell activity, and thus blockade of CD96 in order to induce anti-tumor immune responses is investigated for potential immunotherapeutic intervention in cancer (7-9). Alternative splicing occurs at this locus, and two transcript variants encoding distinct isoforms with variable functions have been identified (1,10). Alternate Names CD96; CD96 antigen; CD96 molecule; Cell surface antigen CD96; DKFZp667E2122; MGC22596; T cell activation, increased late expression; T cell-activated increased late expression protein; T-cell surface protein tactile; TACT; TACTILE Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 100, 160 Source/Isotype: Rabbit IgG