CST, 38519S, Toll-like Receptor 4 (E5D8T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TLR4. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Toll-like Receptor 4 (E5D8T) Rabbit Monoclonal Antibody (CST #38519) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Toll-like Receptor 4 (E5D8T) Rabbit Monoclonal Antibody recognizes endogenous levels of total TLR4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human TLR4 protein. Background Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in , play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adapter proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adapter-like/TIR-associated protein (MAL/TIRAP), TIR domain-containing adapter-inducing IFN-β (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm. Toll-like receptor 4 (TLR4) functions in association with MD-2 in the recognition and initiation of immune responses elicited by lipopolysaccharides (LPS) of Gram-negative bacteria (4-8). TLR4 triggers the activation of NF-κB, IRF-3, and MAPK pathways, leading to the production of inflammatory cytokines (9). Alternate Names ARMD10; CD284; homolog of Drosophila toll; hToll; TLR-4; TLR4; TOLL; toll like receptor 4; toll like receptor 4 protein; Toll-like receptor 4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120-140 Source/Isotype: Rabbit IgG