CST, 3856S, ILK1 (4G9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ILK1. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, ILK1 (4G9) Rabbit Monoclonal Antibody (CST #3856) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 53303 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity ILK1 (4G9) Rabbit Monoclonal Antibody detects endogenous levels of total ILK1 protein. This antibody may cross-react with ILK2. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Zebrafish, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human ILK1. Background Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility (1). ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin (1). ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin (2-5). These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP (1,3,6). It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1 (2). Alternate Names 59 kDa serine/threonine-protein kinase; DKFZp686F1765; epididymis secretory protein Li 28; HEL-S-28; ILK; ILK-1; ILK-2; ILK1; ILK2; integrin linked kinase; integrin-linked kinase; integrin-linked kinase-2; Integrin-linked protein kinase; P59; p59ILK Specification REACTIVITY: H M R Hm Mk Z B Pg SENSITIVITY: Endogenous MW (kDa): 51 Source/Isotype: Rabbit IgG