CST, 3864S, Phospho-FRS2-alpha (Tyr196) Antibody

Polyclonal Antibody for studying FRS2 (Tyr196) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-FRS2-alpha (Tyr196) Antibody (CST #3864) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-FRS2-alpha (Tyr196) Antibody detects endogenous levels of FRS2-α only when phosphorylated at tyrosine 196. This antibody may also detect a non-specific band at 65kDa. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr196 of human FRS2-α. Antibodies are purified by protein A and peptide affinity chromatography. Background Fibroblast growth factor receptor substrate 2 (FRS2, also called Suc-associated neurotrophic factor-induced tyrosine-phosphorylated target or SNT) participates in the transmission of extracellular signals from the fibroblast growth factor receptor (FGFR). FGFR activation leads to tyrosine phosphorylation of FRS2 (1). Two FRS2 family members have been identified, FRS2-α (SNT1) and FRS2-β (SNT2) (2), which are phosphorylated by these receptor tyrosine kinases (RTKs). Once phosphorylated, they recruit SH2 domain-containing proteins, including Grb2 and SHP-2 (3,4), mediating downstream signaling. Tyr436 is required for efficient SHP-2 recruitment (5), whereas Tyr196 functions as a docking site for Grb2-Sos complexes (6). Alternate Names epididymis secretory sperm binding protein; FGFR signalling adaptor; FGFR substrate 2; FGFR-signaling adaptor SNT; Fibroblast growth factor receptor substrate 2; FRS1A; FRS2; FRS2A; FRS2alpha; SNT; SNT-1; SNT1; Suc1-associated neurotrophic factor target 1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 85 SOURCE: Rabbit