CST, 38650S, TOP1 (E4Z1Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TOP1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, TOP1 (E4Z1Q) Rabbit Monoclonal Antibody (CST #38650) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TOP1 (E4Z1Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total TOP1 protein. A cross-reactive band of unknown origin is observed around 20 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TOP1 protein. Background DNA topoisomerases I and II are nuclear enzymes; type II consists of two highly homologous isoforms: topoisomerase IIα and IIβ. These enzymes regulate the topology of DNA, maintain genomic integrity, and are essential for processes such as DNA replication, recombination, transcription, and chromosome segregation by allowing DNA strands to pass through each other (1). Topoisomerase I nicks and rejoins one strand of the duplex DNA, while topoisomerase II transiently breaks and closes double-stranded DNA (2). Topoisomerases are very susceptible to various stresses. Acidic pH or oxidative stress can convert topoisomerases to DNA-breaking nucleases, causing genomic instability and cell death. DNA-damaging topoisomerase targeting drugs (e.g., etoposide) also convert topoisomerases to nucleases, with the enzyme usually trapped as an intermediate that is covalently bound to the 5+ end of the cleaved DNA strand(s). Research studies have shown that this intermediate leads to genomic instability and cell death. Thus, agents that target topoisomerases are highly sought after cancer chemotherapeutic drugs (3). Ca -regulated phosphorylation of topoisomerase IIα at Ser1106 modulates the activity of this enzyme and its sensitivity to targeting drugs (4). Alternate Names DNA topoisomerase 1; DNA topoisomerase I; Scl-70 antigen; TOP1; TOPI; topoisomerase (DNA) I; type I DNA topoisomerase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG