CST, 3865S, IL-1RA (20D8) Mouse Monoclonal Antibody

Monoclonal Antibody for studying IL1RA. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, IL-1RA (20D8) Mouse Monoclonal Antibody (CST #3865) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IL-1RA (20D8) Mouse Monoclonal Antibody detects endogenous IL-1RA protein. This antibody does not cross-react with other related proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human IL-1RA protein. Background The founding members of the interleukin-1 (IL-1) superfamily include pro-inflammatory cytokines IL-1α and IL-1β, and a third protein that acts as an IL-1 receptor antagonist (IL-1RA). At least six similar proteins have been recently identified, including a homolog of IL-1RA (IL1F5). The three better-characterized proteins (IL-1α, IL-1β, and IL-1RA) are mainly expressed in macrophages, monocytes, and dendritic cells. IL-1α and IL-1β act as potent inflammatory cytokines that help regulate host defense and immune responses (1). Binding of these pro-inflammatory cytokines to an IL-1 receptor recruits adaptor proteins (such as IRAK) to the receptor. Phosphorylation of these adaptor proteins promotes downstream signaling cascades associated with the immune response (2). Altered expression of both IL-1α and IL-1β is associated with an extensive list of human disorders, including Alzheimer's disease, rheumatoid arthritis, psoriasis, and various forms of cancer (3,4). IL-1RA acts as an anti-inflammatory cytokine, binding the IL-1 receptor to limit the response to inflammation (5). Because it plays a key role in regulating the inflammatory response, recombinant IL-1RA is a therapeutic agent used in the treatment of diseases, such as rheumatoid arthritis. Alternatively, mutation of the corresponding gene may be associated with susceptibility to the development of specific cancers (6). Alternate Names DIRA; ICIL-1RA; IL-1ra; IL-1ra3; IL-1RN; IL1 inhibitor; IL1F3; IL1RA; IL1RN; IL1RN (IL1F3); interleukin 1 receptor antagonist; Interleukin-1 receptor antagonist protein; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra); IRAP; MGC10430; MVCD4; type II interleukin-1 receptor antagonist Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Mouse IgG1