CST, 3866T, Caspase-1 (D7F10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Casp1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Caspase-1 (D7F10) Rabbit Monoclonal Antibody (CST #3866) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 85315 . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Caspase-1 (D7F10) Rabbit Monoclonal Antibody detects endogenous levels of full length human Caspase-1. The activated p20 subunit was detected by over-expression. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues within the p20 subunit of human caspase-1. Background Caspase-1, or interleukin-1ß converting enzyme (ICE/ICEα), is a class I cysteine protease, which also includes caspases -4, -5, -11, and -12. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1ß and interferon-γ inducing factor (IL-18) into their mature forms (1,2). Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10. Caspase-1 has a large amino-terminal pro-domain that contains a caspase recruitment domain (CARD). Overexpression of caspase-1 can induce apoptosis (3). Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1β and are resistant to endotoxic shock (4,5). At least six caspase-1 isoforms have been identified, including caspase-1 α, β, γ, δ, ε, and ζ (6). Most caspase-1 isoforms (α, β, γ, and δ) produce products between 30-48 kDa and induce apoptosis upon overexpression. Caspase-1 ε typically contains only the p10 subunit, does not induce apoptosis, and may act as a dominant negative. The widely expressed ζ isoform of caspase-1 induces apoptosis and lacks 39 amino-terminal residues found in the α isoform (6). Activation of caspase-1 occurs through an oligomerization molecular platform designated the "inflammasome" that includes caspase-5, Pycard/Asc, and NALP1 (7). Alternate Names CASP-1; CASP1; CASP1 nirs variant 1; Caspase 1; caspase 1, apoptosis-related cysteine peptidase; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase); Caspase-1; Caspase-1 subunit p10; Caspase-1 subunit p20; caspase1; Caspase1; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1B-convertase; IL1BC; IL1BCE; interleukin 1-B converting enzyme; interleukin 1-beta convertase; interleukin 1, beta, convertase; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 48, 20 Source/Isotype: Rabbit IgG