CST, 38672SF, SCD1 (C12H5) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SCD. Validated for WB,IHC,IF,IF. Highly specific and rigorously validated in-house, SCD1 (C12H5) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #38672) is ready to ship. Product Usage Information This product is the carrier free version of product #2794. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2794 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity SCD1 (C12H5) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total SCD1 protein. Species cross-reactivity for IHC-P is mouse only. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu35 of mouse SCD1. Background Stearoyl-CoA desaturase 1 (SCD1) is a key lipogenic enzyme found in the endoplasmic reticulum that catalyzes the conversion of palmitoyl-CoA and stearoyl-CoA to palmitoleoyl-CoA (16:1) and oleoyl-CoA (18:1) (1-3). Palmitoleate and oleate are the major components of triglycerides, membrane phospholipids, and cholesterol esters (1). SCD1-knockout mice show improved insulin sensitivity and reduced body fat (1). Disruption of SCD1 in mouse brown adipose tissue strengthens insulin signaling and results in increased translocation of Glut4 to the plasma membrane and enhanced uptake of glucose (4). Furthermore, SCD1 is essential for the onset of diet-induced body weight gain (1) and insulin resistance in the liver (5). Alternate Names ACOD; Acyl-CoA desaturase; Delta-9 desaturase; delta-9-desaturase; Delta(9)-desaturase; FADS5; Fatty acid desaturase; hSCD1; MSTP008; predicted protein of HQ0998; SCD; SCD1; SCDOS; Stearoyl-CoA desaturase; stearoyl-CoA desaturase (delta-9-desaturase); stearoyl-CoA desaturase opposite strand Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG