CST, 38678S, FAR1 (F2Z9W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FAR1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, FAR1 (F2Z9W) Rabbit Monoclonal Antibody (CST #38678) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FAR1 (F2Z9W) Rabbit Monoclonal Antibody recognizes endogenous levels of total FAR1 protein. This antibody does not cross-react with FAR2 protein. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro52 of human FAR1 protein. Background Genome-wide CRISPR-Cas9 suppressor screens show that peroxisomes contribute to ferroptosis in human renal and ovarian carcinoma cells by synthesizing polyunsaturated ether phospholipids (PUFA-ePLs). PUFA-ePLs serve as substrates for lipid peroxidation. Genes identified in the screens include those for alkylglycerone phosphate synthase (AGPS) and fatty acyl-CoA reductase 1 (FAR1), two peroxisomal enzymes catalyzing ether lipid biosynthesis. Studies suggest that AGPS and FAR1 contribute to ferroptosis sensitivity (1,2). In addition, research studies show that FAR1 is essential for ferroptosis mediated by long-chain saturated fatty acids. The expression levels of FAR1 and ferroptosis response are positively correlated in various cancer cell lines (3). Alternate Names DKFZp686A0370; DKFZp686P18247; FACR1; FAR1; fatty acyl CoA reductase 1; Fatty acyl-CoA reductase 1; FLJ22728; FLJ33561; male sterility domain containing 2; Male sterility domain-containing protein 2; MLSTD2; PFCRD; putative fatty acyl reductase; SDR10E1; short chain dehydrogenase/reductase family 10E, member 1 Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 52 Source/Isotype: Rabbit IgG