CST, 38700S, Transportin-1 Antibody

Polyclonal Antibody for studying IPO2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Transportin-1 Antibody (CST #38700) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Transportin-1 Antibody recognizes endogenous levels of total transportin-1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala354 of human transportin-1 protein. Antibodies are purified by peptide affinity chromatography. Background Transportin-1 (TNPO1), also known as MIP or karyopherin-β2, is a nuclear import protein that binds to nuclear localization signals (NLSs) on various proteins to help shuttle them from the cytoplasm to the nucleus (1-3). While initially discovered to shuttle hnRNP proteins, transportin-1 has also been shown to import other important regulatory proteins, such as c-Fos and ADAR1 (1,4,5). Transportin-1 plays a role in HIV replication, as it imports and helps uncap the capsid protein (6). Transportin-1 has been implicated in familial ALS, due to its impaired binding affinity to mutations in the FUS-NLS region. Reduction in transportin-1 binding correlated with the time of ALS disease progression in these mutants (7). Transportin-1 can also accumulate in FUS inclusions in wild-type FUS ALS (8). Additionally, transportin-1 plays a role in circadian rhythms by importing PER1 to the nucleus (9). Alternate Names importin 2; importin beta 2; Importin beta-2; IPO2; karyopherin (importin) beta 2; Karyopherin beta-2; KPNB2; M9 region interaction protein; MIP; MIP1; TNPO1; transportin 1; Transportin-1; TRN Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 95 SOURCE: Rabbit