CST, 38734S, ZBP1 (E6H8F) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying ZBP1 mouse. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, ZBP1 (E6H8F) Rabbit Monoclonal Antibody (PE Conjugate) (CST #38734) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity ZBP1 (E6H8F) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total ZBP1 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro266 of mouse ZBP1 protein. Background ZBP1 (Z-DNA binding protein 1), also referred to as DAI (DNA-dependent activator of IFN-regulatory factors) and DLM-1, is a nucleotide binding protein that plays a role in tumorigenesis and innate immune responses to viral infection (1). It is expressed at high levels in lymphatic tissues and intestine and is induced in macrophages by interferon gamma or by LPS (2,3). It contains two amino-terminal Z-alpha domains that bind to left-handed Z-DNA and Z-RNA (4,5). In addition, an adjacent domain binds right-handed B-DNA that allows for it to function as a cytosolic DNA sensor in innate immune responses, triggering activation of TBK1 and IRF-3, and subsequent production of type I interferons (6,7). Furthermore, ZBP1 can trigger the activation of NF-κB through interaction with the RIP homotypic interaction motif (RHIM) of RIPK1 and RIPK3 (8). ZBP1 binding to RIPK3 can also induce a process of programmed necrosis termed necroptosis (9). In contrast, its interaction with RIPK1 can repress necroptosis (10,11). The mRNA binding activity of ZBP1 is also thought to play a role in tumorigenesis. ZBP1 is repressed in metastatic breast cancer, which leads to dysregulation of mRNA targets involved in proliferation and metastasis (12,13). Alternate Names 2010010H03Rik; Dai; Dlm1; DNA-dependent activator of IFN-regulatory factors; mZaD; mZaDLM; OTTMUSP00000018050; Tumor stroma and activated macrophage protein DLM-1; Z-DNA binding protein 1; Z-DNA-binding protein 1; Zbp1 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG