CST, 38774S, CD28 (D2Z4E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD28. Validated for WB,IP,IHC,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CD28 (D2Z4E) Rabbit Monoclonal Antibody (CST #38774) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 IHC Leica Bond: 1:400 - 1:1600 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 87823 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity CD28 (D2Z4E) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD28 protein and is predicted to react with multiple CD28 isoforms that are produced through alternative splicing. This antibody cross-reacts with 16 kDa and 100 kDa proteins of unknown origin in some mouse cells. Non-specific staining was observed in mouse testis by immunohistochemistry. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro208 of human CD28 protein. Background CD28 is a transmembrane glycoprotein expressed by T cells as well as some other hematopoietic cells (1,2). T cell activation requires T cell receptor (TCR) recognition of antigen presented in the context of MHC molecules. CD28 acts as a T cell costimulatory receptor, and interaction of CD28 with its ligands CD80 or CD86 provides the second signal required for naïve T cell activation (3-5). Activation of naïve T cells in the absence of CD28 stimulation can result in a state of T cell anergy, or unresponsiveness (3). CD28 signals through cytoplasmic phospho-tyrosine motifs that bind several SH2 or SH3 domain-containing proteins involved in T cell activation (2). Recently, CD28 was demonstrated to be a preferred target of PD-1-mediated dephosphorylation. Consistently, CD28 expression was required for T cell proliferation following PD-1 blockade and CD28 stimulation was required for effective anti-PD-1 cancer immunotherapy in mice (6,7). Several CD28 isoforms are produced by alternative splicing (8). Alternate Names CD28; CD28 antigen; CD28 molecule; MGC138290; T-cell-specific surface glycoprotein CD28; TP44 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 14, 40-60 Source/Isotype: Rabbit IgG