CST, 38775SF, FoxA2/HNF3 beta (D56D6) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying FOXA2. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, FoxA2/HNF3 beta (D56D6) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #38775) is ready to ship. Product Usage Information This product is the carrier free version of product #8186. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8186 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity FoxA2/HNF3 beta (D56D6) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total FoxA2/HNF3β protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly138 of human FoxA2/HNF3β protein. Background Forkhead box protein A2 (FoxA2, also known as hepatocyte nuclear factor 3β or HNF3β) is a transcription factor that plays an important role in hepatocyte function (1). FoxA2/HNF3β is required for the activation of hepatic gluconeogenic gene expression during fasting (1). Together with the PGC-1β coactivator, FoxA2/HNF3β stimulates the expression of genes involved in fatty acid β-oxidation and, therefore, increases fatty acid metabolism (2). FoxA2/HNF3β, along with PGC-1β, also activates the expression of microsomal triacylglycerol transfer protein (MTP) and promotes VLDL secretion (2). In addition to its roles in metabolic syndromes, FoxA2/HNF3β is essential for development of the endoderm and midline structures in mouse embryos (3-5). Alternate Names forkhead box A2; Forkhead box protein A2; FOXA2; hepatic nuclear factor-3-beta; Hepatocyte nuclear factor 3-beta; hepatocyte nuclear factor 3, beta; HNF-3-beta; HNF-3B; HNF3B; MGC19807; TCF-3B; TCF3B; Transcription factor 3B Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG