CST, 3884S, Phospho-Gab2 (Ser159) Antibody

Polyclonal Antibody for studying Gab2 (Ser159) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Gab2 (Ser159) Antibody (CST #3884) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Gab2 (Ser159) Antibody detects endogenous levels of Gab2 only when phosphorylated at serine 159. This antibody does not cross-react with Gab1 or Gab3. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser159 of human Gab2. Antibodies are purified by protein A and peptide affinity chromatography. Background The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9). Akt phosphorylates Gab2 at Ser159. This phosphorylation inhibits Gab2 tyrosine phosphorylation and downstream signal amplification (10). Alternate Names GAB2; GRB2 associated binding protein 2; GRB2-associated binder 2; GRB2-associated binding protein 2; GRB2-associated-binding protein 2; Growth factor receptor bound protein 2-associated protein 2; KIAA0571; pp100 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 98 SOURCE: Rabbit