CST, 3885S, ERCC1 Antibody

Polyclonal Antibody for studying ERCC1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, ERCC1 Antibody (CST #3885) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ERCC1 Antibody recognizes endogenous levels of total ERCC1 isoforms 1, 2 and 4 protein. Experiments utilizing overexpression constructs indicated that this antibody does not cross-react with ERCC1 isoform 3. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human ERCC1. Antibodies are purified by protein A and peptide affinity chromatography. Background DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions occur, such as those brought about by UV and certain chemicals (1). Excision Repair Cross Complementing 1 (ERCC1) forms a complex with ERCC4/XPF, which acts as the 5' endonuclease required to excise the lesion (2). ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) (3) and involved in repair of double strand breaks (4). Research studies have shown that expression of ERCC1 is related to survival rate and response to chemotherapeutic drugs in several human cancers including non-small cell lung cancer (NSCLC) (5,6). Alternate Names COFS4; DNA excision repair protein ERCC-1; ERCC excision repair 1, endonuclease non-catalytic subunit; ERCC1; excision repair cross-complementation group 1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence); RAD10; UV20 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 39 SOURCE: Rabbit