CST, 38910T, TRIM16 (F3S3I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TRIM16. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, TRIM16 (F3S3I) Rabbit Monoclonal Antibody (CST #38910) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity TRIM16 (F3S3I) Rabbit mAb recognizes endogenous levels of total TRIM16 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala69 of human TRIM16 protein. Background Misfolded proteins are cleared in cells by ubiquitination and degradation using the proteasome and autophagy pathways (1-5). Various factors, including mutations, oxidative stress, and environmental conditions, can trigger increased protein misfolding and overwhelm cells, resulting in aggregation, accumulation, and disease (2,3). Tripartite Motif-Containing Protein 16 (TRIM16) was first discovered to regulate the NRF2 pathway (3) along with other autophagy proteins, such as Beclin-1, ULK1, and ATG16L, helping to facilitate protein aggregate clearing (1-3,6). TRIM16 plays an important role in cellular defense against oxidative stress and autophagy (4-6). The TRIM family of proteins is highly conserved and has been implicated in a diverse range of biological processes, including development, cell growth, differentiation, innate immune functions, and cancer (4). TRIMs most commonly have a RING finger domain, B-box domains, a coiled-coil domain, and a variable C-terminal domain (4). Specifically, TRIM16 lacks a RING domain but possesses two B-box domains and a C-terminal SPRY domain. TRIM16 has recently been shown to function as an E3 ubiquitin ligase (1,4). TRIM16 is also studied as a tumor suppressor, influencing cell differentiation and migration, and has been implicated in various cancers (7-9), including pancreatic, bladder (9), and breast cancer (7). Alternate Names E3 ubiquitin-protein ligase TRIM16; EBBP; Estrogen-responsive B box protein; TRI16; TRIM16; tripartite motif containing 16; tripartite motif-containing 16; Tripartite motif-containing protein 16 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG