CST, 3894S, PARN (A42) Antibody

Polyclonal Antibody for studying PARN. Validated for Western Blotting. Highly specific and rigorously validated in-house, PARN (A42) Antibody (CST #3894) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PARN (A42) Antibody detects endogenous levels of total PARN protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequence surrounding Ala42 of human PARN. Antibodies are purified by Protein A and peptide affinity chromatography. Background Cellular levels of mRNAs are controlled by mRNA stability, the rate of synthesis and the rate of degradation. The presence and length of the poly(A) tail has been associated with mRNA stability (1). Exonucleolytic shortening of the poly(A) tail is the process that initiates the decay of many eukaryotic mRNAs (2). Poly(A)-specific ribonuclease (PARN) is the enzyme responsible for initiation of deadenylation and exonucleolytic shortening of mRNA transcripts. Through an evolutionarily conserved mechanism, PARN also translationally silences selective mRNAs during early embryonic development (3). PARN is constitutively expressed in most mammalian tissues and plays a critical role in the post-transcriptional control of gene expression (4). Alternate Names DAN; Deadenylating nuclease; Deadenylation nuclease; DKCB6; PARN; PFBMFT4; poly(A)-specific ribonuclease; poly(A)-specific ribonuclease (deadenylation nuclease); Poly(A)-specific ribonuclease PARN; Polyadenylate-specific ribonuclease Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 78 SOURCE: Rabbit