CST, 3904S, G-alpha(z) Antibody

Polyclonal Antibody for studying G-alpha(z). Validated for Western Blotting. Highly specific and rigorously validated in-house, G-alpha(z) Antibody (CST #3904) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity G-alpha(z) Antibody detects endogenous levels of total Gα(z) protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human Gα(z). Antibodies are purified using protein A and peptide affinity chromatography. Background Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines, and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP formation (3). Gα(z) stands out from other G proteins because it lacks an ADP-ribosylation consensus site for pertussis toxin, giving it a possible role in signal transduction pathways resistant to the toxin, such as phospholipase C (4). Gα(z) is phosphorylated and activated by protein kinase C (PKC) at Ser27 (5,6). Alternate Names G protein subunit alpha z; G-alpha(z); G(x) alpha chain; GNAZ; guanine nucleotide binding protein (G protein), alpha z polypeptide; Guanine nucleotide-binding protein G(z) subunit alpha; Gz-alpha; transducin alpha Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit