CST, 3909S, UTF1 Antibody

Polyclonal Antibody for studying UTF1. Validated for Western Blotting. Highly specific and rigorously validated in-house, UTF1 Antibody (CST #3909) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity UTF1 Antibody detects endogenous levels of total UTF1 protein. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide correponding to amino acid sequence near the C-terminus of human UTF1. Antibodies are purified by Protein A and peptide affinity chromatography. Background Undifferentiated embryonic cell transcription factor 1 (UTF1) is expressed in cells of the inner cell mass and the epiblast (1). Expression is down-regulated with development, although it is maintained in the embryonic germ cells and in the adult gonads (1). Reduced expression in embryonic stem cells (ESCs) is associated with failure to differentiate properly, although self-renewal is unaffected (2). UTF1 is tightly associated with chromatin in mouse and human ESCs and may be involved in maintaining an epigenetic environment necessary for the pluripotent state (2,3). Co-expression of UTF1 with reprogramming factors c-Myc, Oct-4, Sox2 and KLF4, along with siRNA knock-down of p53 increased efficiency of induced pluripotent stem cell generation by 100 fold (4). Alternate Names Undifferentiated embryonic cell transcription factor 1; UTF1 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit