CST, 3912S, p62Dok Antibody

Polyclonal Antibody for studying Dok1. Validated for Western Blotting. Highly specific and rigorously validated in-house, p62Dok Antibody (CST #3912) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity p62Dok Antibody detects transfected levels of p62Dok proteins. This antibody does not cross-react with related proteins. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr398 of human p62Dok. Antibodies are purified by protein A and peptide affinity chromatography. Background p62Dok (Dok-1) is a major tyrosine-phosphorylated, GAP-associated, 60 kDa protein present within the cells transformed by different tyrosine kinases (1). p62Dok contains an amino-terminal pleckstrin homology domain potentially involved in phospholipid interaction and membrane targeting, a central putative phospho-tyrosine binding domain for interacting with tyrosine-phosphorylated proteins. There are numerous tyrosines in its carboxy-terminal region that are potential targets for tyrosine kinases. If phosphorylated, these tyrosines could serve as docking sites for proteins that contain an SH2 domain (2). Overexpression of p62Dok has been shown to inhibit Ras activity in human embryonic kidney 293 cells and B cell antigen receptor-mediated c-Fos promoter activation in an immature B cell line (3), suggesting that p62Dok may play a negative role in Ras signaling. Moreover, p62Dok overexpression may also inhibit insulin-stimulated Akt activation (4). Alternate Names Docking protein 1; docking protein 1 (downstream of tyrosine kinase 1); docking protein 1, 62kDa (downstream of tyrosine kinase 1); DOK1; Downstream of tyrosine kinase 1; MGC117395; MGC138860; p62(dok); P62DOK; pp62 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 62 SOURCE: Rabbit