CST, 39138S, SNX5 (E4Q2X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SNX5. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SNX5 (E4Q2X) Rabbit Monoclonal Antibody (CST #39138) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SNX5 (E4Q2X) Rabbit Monoclonal Antibody recognizes endogenous levels of total SNX5 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu216 of human SNX5 protein. Background Sorting nexins (SNXs) are a family of cytoplasmic proteins characterized by the presence of a phosphatidylinositol 3-phosphate (PI3P) binding phox (PX) domain. This binding occurs mainly in the early endosome and allows for trafficking of the bound protein to either a degradative or recycling pathway (1). The sorting nexin family member SNX5 was identified in binding studies with Fanconi anemia complementation group A (FANCA) protein (2). In addition to the PX domain, STX5 has a Bin/Amphiphysin/Rvs (BAR) domain that can sense membrane curvature and induce membrane tubulation (3). Aberrant expression of SNX5 can contribute to tumorigenesis, invasion, and metastasis of several human cancers (4-7). Elevated expression of SNX5 has been reported in Parkinson's disease, where it may have a role in promoting ferroptosis (8). Studies have found that SNX5 has an essential role in macropinocytosis (9). In addition, SNX5 is part of a complex involved in recognizing membrane curvature on autolysosomes and regulating recycling of autophagosomal components (10). In a genome-wide siRNA, SNX5 was found to be an essential protein involved in virus-mediated autophagy and immunity (11). Alternate Names FLJ10931; SNX5; sorting nexin 5; Sorting nexin-5 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 47 Source/Isotype: Rabbit IgG