CST, 3913S, Tropomyosin-1/3 (D17B8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TPM1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Tropomyosin-1/3 (D17B8) Rabbit Monoclonal Antibody (CST #3913) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Tropomyosin-1/3 (D17B8) Rabbit Monoclonal Antibody detects endogenous levels of total tropomyosin-1 and/or tropomyosin-3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu274 of human tropomyosin-1 protein. Background Tropomyosin-1 (TPM1) belongs to the high molecular weight members of tropomyosin family (1,2). The protein exists in an alpha-helical coiled-coil conformation and binds multiple acting monomers in a tight manner to stabilize and regulate the actin filament (3). Tropomyosins fullfill functions in muscle and non-muscle cells. In muscle cells, tropomyosins associate with the troponin complex and play a central role in the calcium-dependent regulation of striated muscle contraction in vertebrates. In non-muscle cells, tropomyosins are implicated in the formation and stabilization of cytoskeletal actin filaments to ensure normal cellular processes (1,2). Mutations of tropomysin-1 have been reported as a cause of dilated cardiac myopathies (4). Tropomyosin-1 also functions as a tumor suppressor, and many malignant tumors demonstrate downregulation of tropomyosin-1 expression (5-8). Tropomyosin-1 is phosphorylated at Ser283 through the Erk/DAPK pathway, which promotes stress fiber formation in response to oxidative stress (9-10). Alternate Names alpha tropomyosin; Alpha-tropomyosin; C15orf13; cardiomyopathy, hypertrophic 3; CMD1Y; CMH3; epididymis secretory protein Li 265; HEL-S-265; HTM-alpha; LVNC9; sarcomeric tropomyosin kappa; TMSA; TPM1; tropomyosin 1; tropomyosin 1 (alpha); Tropomyosin alpha-1 chain; Tropomyosin-1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 32 , 35, 36, 41 Source/Isotype: Rabbit IgG