CST, 39143SF, CD11c (D1V9Y) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ITGAX mouse. Validated for WB,IHC,IHC,IF,IF. Highly specific and rigorously validated in-house, CD11c (D1V9Y) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #39143) is ready to ship. Product Usage Information This product is the carrier free version of product #97585. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 97585 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD11c (D1V9Y) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD11c protein. Non-specific staining of the epithelium in fixed frozen mouse intestine was observed by immunofluorescence. Non-specific staining in fixed frozen mouse brain was observed by immunofluorescence. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1153 of mouse CD11c protein. Background CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein that forms an α/β heterodimer with CD18 (integrin β2), which interacts with a variety of extracellular matrix molecules and cell surface proteins (1). CD11c is primarily used as a dendritic cell marker. Dendritic cells can be classified into two major types: CD11c+ conventional dendritic cells that specialize in antigen presentation, and CD11c- plasmacytoid dendritic cells that specialize in type I interferon production (2, 3). CD11c expression has also been observed on activated NK cells, subsets of B cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (4-7). Alternate Names AI449405; CD11 antigen-like family member C; Cd11c; CD11C (p150) alpha polypeptide; complement receptor 4; Cr4; integrin alpha X; Integrin alpha-X; integrin aX; ITAX; Itgax; leukocyte adhesion glycoprotein p150,95 alpha chain; leukocyte adhesion receptor p150,95; mFLJ00114; MFLJ00114 protein; N418 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 145 Source/Isotype: Rabbit IgG