CST, 3920T, Bag1 (3.10G3E2) Mouse Monoclonal Antibody

Monoclonal Antibody for studying BAG1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Bag1 (3.10G3E2) Mouse Monoclonal Antibody (CST #3920) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Bag1 (3.10G3E2) Mouse Monoclonal Antibody detects endogenous levels of total Bag1 protein, including long, medium and short isoforms. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant full length human Bag1. Background Bag1 belongs to the Bcl-2 associated athanogene (BAG) family of multifunctional proteins and was the first of six related proteins isolated from humans (1,2). This widely expressed protein interacts with a number of signaling molecules (including Bcl2, HGF receptor and Raf1) as it regulates signaling molecules in pathways involving cell survival, growth and differentiation. The most common role played by Bag1 protein is as an inhibitor of proteins favoring apoptosis (2-4). Bag1 also plays a role in Raf1 signaling and binds DNA as a transcription activator (4). Bag1 protein is a well-characterized inhibitor of its binding partner HSP70 (5). A conserved carboxy-terminal BAG domain within Bag1 interacts with the ATPase domain of HSP70 to negatively regulate heat shock protein chaperone activity (6,7). The multiple isoforms of Bag1 protein generated from a single transcript share a common ubiquitin homology domain and a carboxy-terminal Hsp70 binding region but differ in length and cellular localization. The 50 kDa long (Bag1-L) isoform also contains a nuclear localization signal and is often found in the nucleus where it activates transcription. The 46 kDa intermediate (Bag1-M) isoform is found mainly in the cytoplasm but can also translocate to the nucleus when associated with other proteins. The shorter 29-33 kDa isoforms (Bag1-S, Bag-1) isoforms are found primarily in the cytoplasm (8). High expression of the anti-apoptotic Bag1 protein correlates with increased survival in patients with particular forms of cancer, leading researchers to study possible therapeutic roles for Bag1 protein (9). Alternate Names BAG cochaperone 1; BAG family molecular chaperone regulator 1; BAG-1; BAG1; Bcl-2 associating athanogene-1 protein; Bcl-2-associated athanogene 1; Bcl-2-binding protein; BCL2 associated athanogene 1; BCL2-associated athanogene; glucocortoid receptor-associated protein RAP46; HAP; RAP46; receptor-associated protein, 46-KD Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 52, 46, 33 Source/Isotype: Mouse IgG1