CST, 3922S, IGFBP2 Antibody

Polyclonal Antibody for studying IGFBP-2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, IGFBP2 Antibody (CST #3922) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity IGFBP2 Antibody detects endogenous levels of total IGFBP2 protein. This antibody does not cross-react with other IGFBP family members. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the residues at the carboxy-terminal sequence of human IGFBP2. The antibodies are purified by protein A and peptide affinity chromatography. Background Insulin-like growth factor-binding proteins (IGFBPs) play an integral role in modifying insulin-like growth factor (IGF) actions in various cell types. This family contains six members that are structurally related but encoded by distinct genes. IGFBPs have a high affinity for IGFs. Some IGFBP family members have been consistently shown to inhibit IGF actions by preventing them from gaining access to IGF receptors, while others potentiate IGF actions by facilitating the ligand-receptor interaction (1-3). IGFBP2 is the second most abundant IGFBP in the circulation and is present in various other biological fluids and tissues of many vertebrate species. Serum IGFBP2 levels are elevated in conditions such as shock, fasting, hypoxemia, or after traumata, suggesting complex regulation of IGFBP2 expression (4). IGFBP2 is overexpressed in many malignancies and is often correlated with an increasingly malignant tumor status, pointing to a potential involvement of IGFBP2 in tumorigenesis (5). Alternate Names BP2; IBP-2; IBP2; IGF-binding protein 2; IGF-BP53; IGFBP-2; IGFBP2; insulin like growth factor binding protein 2; insulin-like growth factor binding protein 2, 36kDa; Insulin-like growth factor-binding protein 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35 SOURCE: Rabbit