CST, 39263S, DOCK8 (E5Y9C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DOCK8. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DOCK8 (E5Y9C) Rabbit Monoclonal Antibody (CST #39263) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DOCK8 (E5Y9C) Rabbit Monoclonal Antibody recognizes endogenous levels of total DOCK8 protein. This antibody recognizes DOCK8 isoforms 1-4. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DOCK8 protein. Background DOCK8 (dedicator of cytokinesis 8) is a member of the DOCK family of proteins that function as guanine nucleotide exchange factors (GEFs) for the Rho family of GTPases (1,2). DOCK8 is responsible for activation of the GTPase Cdc42, which exchanges bound GDP for free GTP, and plays a regulatory role in cell polarity and migration (2). DOCK8 immunodeficiency syndrome, or autosomal-recessive hyper-IgE syndrome, is caused by mutations of (3,4). Patients with this disorder have susceptibility to viral infections, eczema, increased serum IgE, defective T cell activation and differentiation, and impaired eosinophil homeostasis, due to T cell dysfunction associated with DOCK8 deficiency (2-4). Alternate Names 1200017A24Rik; dedicator of cytokinesis 8; Dedicator of cytokinesis protein 8; DOCK8; epididymis luminal protein 205; FLJ00026; FLJ00152; FLJ00346; HEL-205; MRD2; ZIR8 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 220-240 Source/Isotype: Rabbit IgG