CST, 39275T, CD69 (E1U2O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD69. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CD69 (E1U2O) Rabbit Monoclonal Antibody (CST #39275) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD69 (E1U2O) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD69 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu120 of human CD69 protein. Background CD69, also known as Leu-23, is a type II transmembrane glycoprotein that is expressed on the surface of T cells, B cells, and NK cells (1,2). This phosphorylated disulfide-linked 28 to 32-kDa homodimer is constitutively expressed on a subset of thymocytes and platelets. It also acts as an activation antigen of lymphocytes, NK cells, neutrophils, and eosinophils (1-6). Studies have shown that stimulation of the T cell receptor (TCR) increases the expression of CD69 on the cell surface. The ability to detect the level of CD69 expression after TCR activation makes CD69 an ideal indicator of T cell activation (1). Alternate Names Activation inducer molecule; activation inducer molecule (AIM/CD69); AIM; BL-AC/P26; C-type lectin domain family 2 member C; C-type lectin domain family 2, member C; CD69; CD69 antigen (p60, early T-cell activation antigen); CD69 molecule; CLEC2C; EA1; Early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 25-40 Source/Isotype: Rabbit IgG